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培養細胞に蓄積された重金属化合物のエネルギー分散型分析電子顕微鏡による検出


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Title: 培養細胞に蓄積された重金属化合物のエネルギー分散型分析電子顕微鏡による検出
Other Titles: Detection of Heavy Metal Compounds Accumulated in Cultured Cells using Energy Disperse Type Analytical Electron Microscope
Authors: 一ノ瀬, 昭豊 / 五十嵐, 章
Authors (alternative): Ichinose, Akitoyo / Igarashi, Akira
Issue Date: 30-Sep-1985
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 27(3). p181-190, 1985
Abstract: Presence of heavy metal compounds, such as mercury, chromium, and lead, were detected in cultured mammalian cells, LLC-MK2, by means of energy disperse type electron microscope. Introduction of these heavy metal compounds at high concentrations from 1 to 10 mM in the culture medium, resulted in the morphological change of cultured cells after 2 days of incubation at 37℃. The change was accompanied by the appearance of intracytoplasmic electron-dense granules from which significant levels of metal-specific signals were recorded. On the other hand, no significant metal-specific signals were recorded in the specimens when the cells were maintained for 2 days under media containing less than 0.1 mM of these metal compounds. The results indicated the capacity and limitation of the equippment to detect the accumulation of the heavy metals in medical or biological specimens.
URI: http://hdl.handle.net/10069/4418
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 27, No. 3

Citable URI : http://hdl.handle.net/10069/4418

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