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日本とタイ国で分離された日本脳炎ウイルス株間のペプチドマッピングによる比較


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Title: 日本とタイ国で分離された日本脳炎ウイルス株間のペプチドマッピングによる比較
Other Titles: Comparison of Japanese Encephalitis Virus Isolates from Japan and Thailand by Peptide Mapping
Authors: SRIVASTAVA, Ashok Kumar / 五十嵐, 章
Authors (alternative): Srivastava, Ashok Kumar / Igarashi, Akira
Issue Date: 28-Dec-1985
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 27(4). p269-275, 1985
Abstract: 日本とタイ国で分離された日本脳炎ウイルス株をSDSポリアクリルアミドゲル電気泳動により分析した,検査したすべての株において三種の構造タンパクV1,V2,V3を認めた.黄色ブドウ球菌V8タンパク分解酵素によって中山株のV3タンパクは他の株のものよりも容易に加水分解された.より高濃度のV8タンパク分解酵素を用いるとすべての株のV3タンパクは加水分解されて類似のペプチドパターンを示した. / Purified preparations of Japanese encephalitis (JE) virus isolates from Japan and Thailand were analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) to reveal their structural proteins. Three structural polypeptides, V1, V2 and V3, were observed with all the strains under reducing condition. Limited digestion of V3 by Staphylococcus aureus V8 protease showed that Nakayama strain was more easily cleaved than other isolates. At higher concentration of the protease, V3 structural protein of all the strains were cleaved with similar peptide pattern.
URI: http://hdl.handle.net/10069/4428
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 27, No. 4

Citable URI : http://hdl.handle.net/10069/4428

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