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Title: ウエスタンプロット法による子宮頸癌患者と健常人血清中の2型単純ヘルペスウイルス(HSV-2)抗体の解析とELISA法による抗体価測定
Other Titles: Western Blotting Analysis and ELISA on Serum Antibodies against Herpes Virus Type-2 (HSV-2) among Uterine Cervical Cancer Patients and Healthy Women
Authors: 前田, 由紀子
Authors (alternative): Maeda, Yukiko
Issue Date: 30-Sep-1986
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 28(3). p165-178, 1986
Abstract: Antibodies against HSV-2 early antigens (EA) prepared from infected HEp-2 cells were analyzed by the Western blotting method using 20 each individual sera from uterine cervical cancar (CaCx) patients as well as from healthy control women. The number of polypeptide bands reacted with CaCx patients' sera were generally more than that with healthy controls, especially for IgM than IgG-class of antibodies. IgM antibodies against 3 bands with estimated molecular weight of 110k, 74k, and 64k daltons were more frequently detected among CaCx patients than controls with statistical significance level of 1%. While, IgG antibodies against 100k dalton band were more frequent among CaCx patients than controls with statistical significance level of 5%. The 110k and 64k dalton polypeptides were more abundant in nuclear fraction, while 74k dalton polypeptide was more in the cytoplasm. The EA primarily containing 110k and 64k dalton polypeptides was prepared from nuclear fraction of HSV-2 infected HEp-2 cells after 12 hours of incubation in the presence of cytosine arabinoside (Ara-C) and was used to measure IgG and IgM antibody titers among CaCx patients, other cancer patients as well as healthy controls by the indirect micro ELISA. Antibody titers against HSV-2 virion antigen was also measured and compared by the same procedure. Significant difference was observed between CaCx patients and healthy controls and also between CaCx patients and other cancer patients in their IgM titer distribution against EA at 1% significance level. Percentage of the specimens with IgM titers exceeding the upper limit of healthy controls (360) was 10.8% for CaCx and 1.9% for other cancer patients, while, difference in the IgG titer against EA was observed between CaCx patients and healthy controls at 5% significance level. In contrast, titers against virion antigen showed significant difference at 5% level only in IgG antibodies between CaCx patients and healthy controls. Correlation coefficients and equation of linear regression for each combination of antibodies and antigens indicated some difference between CaCx patients and healthy controls in terms of production of HSV-2 EA and virion, or their antigen recognition process. Follow-up studies on 39 postoperative CaCx cases showed that recurrence was observed among 5 of the 11 cases with significant increase in their IgM-ELISA titers against HSV-2 EA. No recurrence was observed among 28 cases with decreasing or constant IgM-ELISA titers during observation period, although 3 of them showed high IgM titers exceeding the upper limit of 360 for healthy controls. Difference between these 2 groups was statistically significant of less than 0.5% level.
URI: http://hdl.handle.net/10069/4448
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 28, No. 3

Citable URI : http://hdl.handle.net/10069/4448

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