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容量式湿度計-発汗カプセル法による暑熱順化の解析


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Title: 容量式湿度計-発汗カプセル法による暑熱順化の解析
Other Titles: Determination of Heat Acclimatization by Capacitance Hygrometer-Sweat Capture Capsule Method
Authors: 范, 育仁
Authors (alternative): Fan, Yu-Jen
Issue Date: 30-Jun-1987
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 29(2). p107-121, 1987
Abstract: 気温28℃,湿度60%に調節された環境制御実験装置で暑熱地住民や日本人スポーツマンの両下肢に温熱刺激(43℃温水,30分)を負荷して体温調節の熱放散反応(特に温熱発汗)を誘発し,サーモグラフィ装置や容量式湿度計-発汗カプセル法によって発汗開始時間,発汗閾値口腔温,刺激中(20分間),刺激後(5分間)の発汗量などを各種熱放散指標と同時記録・解析して、暑熱地住民・日本人スポーツマン・日本人非スポーツマンの3群についてデーターを比較し暑熱順化の中枢性・末梢性機序解明に資した.本研究では特に(1)容量式湿度計-発汗カプセル法による発汗定量の校正曲線(calibration)の描記に新考案を試み,被験者に装着した発汗カプセルの頂部にもうけた小孔からカプセル下の皮膚の上に汗や30℃・0.45% NaCl溶液を0.01,0.02,0.03,0.04mlの容量ずつマイクロピペットを用いて段階注入,高感度容量湿度計を介して相対湿度(%RH)曲線を連続記録し,これを絶対湿度に変換,最終的には発汗量をmg/cm^2・minの単位で測定し,実記録中の発汗量の計測を可能とした.(2)上記,発汗カプセル法(Fan-Kosaka method)による発汗潜時,発汗開始閾値口腔温はサーモグラフィ装置による胸部・腹部平均皮膚温の変化時点のそれらの一致,さらに容量式湿度計-発汗カプセル法は発汗量の正確な測定が可能である点,発汗解析に極めて有効な手段であることが明らかになった. / Measurements of sweat rate in tropical and Japanese subjects were performed in an environmental control chamber (room temperature 28℃, humidity 60%). Local heat load (43℃ water bath) was applied on lower legs (30min, and 20min) to induce thermal sweating responses. The indicators of thermoregulatory heat loss responses such as sweat onset time, threshold oral temperature for sweating and sweat volumes (for 20 min during heat load, for 5min after heat load) etc. were simultaneously measured by using thermography and capacitance hygrometer-sweat capture capsule method. By analyzing the data of tropical inhabitants and Japanese (sportsmen and non-sportsmen), the central and peripheral mechanism of heat acclimatization were investigated. In this study, a new quantitative calibration by using capacitance hygrometer-sweat capture capsule method was devised, i.e., on the top of the capsule fixed to the skin of subject, a small hole was made, through which, subject's sweat or 30℃ 0.45% NaCl solutions (0.01, 0.02, 0.03 and 0.04 ml) were precisely dropped into the capsule with a micropipette and hole sealed. Relative humidity changes (%RH) of the capsule were continuously recorded by capacitance hygrometer. By calculating the absolute numidity from relative humidity, sweat rate (mg/cm^2・min) could be obtained and sweat volume was quantitatively decided by the sweat rate. (Fan-Kosaka method). Further, it was found that the sweat onset time detected by thermography (i.e., change of skin temperature) and by sweat capture capsule method were consistent. This new method made the measurement of sweat volume simple and accurate, and that these experimental modalities may be utilized in further determination of physiological mechanisms of heat acclimatization.
URI: http://hdl.handle.net/10069/4495
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 29, No. 2

Citable URI : http://hdl.handle.net/10069/4495

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