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NAOSITE : Nagasaki University's Academic Output SITE > Institute of Tropical Medicine > Bulletin > Tropical medicine > Volume 29, No. 3 >

BALB/cマウスにおけるBrugia pahangi感染感受性の性差 : エフェクター細胞の反応の性差について

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Title: BALB/cマウスにおけるBrugia pahangi感染感受性の性差 : エフェクター細胞の反応の性差について
Other Titles: Difference in the Susceptibility to Brugia pahangi Infection between Male and Female BALB/c Mice : Differences of Effector Cell Responses between Sex
Authors: 中西, 弘有
Authors (alternative): Nakanishi, Hirokum
Issue Date: 30-Sep-1987
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 29(3). p153-163, 1987
Abstract: 雄・雌BALB/cマウスにおけるBrugia pahangi感染感受性の性差について検討した.感染幼虫(L3)50隻,マウス腹腔内接種後の虫体回収率の動態を調べてみると,初感染時には感染後比較的早い時期(5-10日)より感受性の性差(雄マウスの回収率は雌マウスに比し高い)が出はじめ,少なくとも感染後7週まで継続した.フィラリア虫体は7-10日という時期に脱皮し虫体の発育ステージを変える事より,いずれのステージの虫体が性差の発現に重要かを検討した.第4期幼虫移入時には性差は認められず,L3移入時のみ性差が認められた.これらの事から,初感染時の性差の発現にはL3(あるいは脱皮ステージの虫体も含む)が最も関与している事が明らかとなった.次に再感染時に性差が出現するか否か検討した.雄・雌マウスとも初感染時に比し著しい虫体回収率の減少を示したが,再感染時の雄・雌マウス間でほ回収率に性差は認められなかった.この事から,マウスにおける性差の発現には初感染時の雄・雌マウス間の虫体に対する比較的早い時期の免疫応答の差が重要な要素であると考えられた.そこで感染後の抗体産生と腹腔内滲出細胞の変動を雄・雌マウス間で比較してみると,性差の出現する時期の5-10日においてはIgG,IgM,IgEの抗体価にほ性差は認められなかったが,腹腔滲出細胞の動態,特にマクロファージ(Mφ)と好酸球(Eos)数には著しい性差が認められた.すなわち,感染抵抗性の強い雌マウスのMφ,Eos数は感染感受性の高い雄マウスに比し有意の増加が認められた.この事からBrugia pahangi初感染初期の性差の発現には抗体よりはMφ,Eosが重要な役割を担っていると考えられた. / Difference in the susceptibility to Brugia pahangi infection between male and female BALB/c mice was examined. In a primary infection, male mice showed greater susceptibility than did females as defined by counting the number of worms recovered from the peritoneal cavity. Sex difference appeared at the early phase of primary infection (5-10 days) and continued for at least 7 weeks of infection. Inoculation with the 3rd stage larvae was effective in inducing sex difference, whereas inoculation with the 4th stage larvae had no effect. Therefore, sex difference in susceptibility seems to be induced by the 3rd stage larvae (possibly molting stage larvae). Since sex difference was only observed in a primary infection but not in a challenge infection, the expression of sex difference might be dependent upon some differences of development of early phase of immune response to the larval worm of B. pahangi between the sex. Thus, in the present study, the difference in immunological response of host to filarial worm was examined. Significantly great number of effector cell infiltrations (macrophages and eosinophils were predominant) were observed in the peritoneal cavities of female mice 10 days after infection. Whereas no significant difference in the levels of anti-B. pahangi IgG, IgM and IgE were observed at this time of infection, the cellular reaction that occured in female mice 10 days postinfection may be one of the factors which reflects the differential sex susceptibility of host in B. pahangi-mouse model.
URI: http://hdl.handle.net/10069/4499
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 29, No. 3

Citable URI : http://hdl.handle.net/10069/4499

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