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プラジカンテルによるSchistosoma mansoniの虫卵の孵化に関する研究

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Title: プラジカンテルによるSchistosoma mansoniの虫卵の孵化に関する研究
Other Titles: Studies on Praziquantel-induced Hatching of the Eggs of Scistosoma mansoni
Authors: 勝又, 達哉
Authors (alternative): Katsumata, Tatsuya
Issue Date: 31-Mar-1988
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 30(1). p13-23, 1988
Abstract: Praziquantel-induced hatching of Schistosoma mansoni eggs was studied. In phosphate buffered saline (PBS) with osmolarity of 365 mOsM or 465 mOsM, most miracidia enclosed within the egg shell were immotile. Once praziquantel (PZQ) was added to PBS to give a final concentration of 1-100 μg/ml, miracidia began to take a screwlike or rotary motion within the shell. And then the egg shell was ruptured and miracidium was released. Thirty-six percent of the mature eggs hatched in PBS (465 mOsM) cotaining PZQ at a concentration of 100 μg/ml during the observation period of 2 hours at 25℃. The corresponding hatching rate of eggs in PBS without PZQ was 8.3%. PZQ-induced hatching was partially inhibited by EGTA (5mM), Lanthanum chloride (0.1-0.5mM) and Ruthenium Red (0.5-1mM). Calcium ionophore (A23187) stimulated the miracidia to swim within the shell and to hatch. Calcium ionophore, at a concentration of 10^<-4> M, allowed 20% of mature eggs to hatch in PBS (365 mOsM) during the observation period of 2 hours. When the eggs were incubated in high concentration of CaCl_2 solution (112mM), the miracidia became motile in some eggs, but they did not hatch. The break of the shell was most frequently located on the long axis of the shell opposite the spine. The edges of the broken shell were sharp, uniform in thickness, and complementary throughout the orifice. The proteinase inhibitors failed to inhibit PZQ-induced hatching. The possible role of Ca^<++> in PZQ-induced hatching process of S. mansoni eggs was disussed.
URI: http://hdl.handle.net/10069/4510
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 30, No. 1

Citable URI : http://hdl.handle.net/10069/4510

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