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犬糸状虫の好中球遊走物質(NCF-Di)のin vivoでの作用と虫体からの放出

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Title: 犬糸状虫の好中球遊走物質(NCF-Di)のin vivoでの作用と虫体からの放出
Other Titles: Direct Attraction of Neutrophils in vivo by the Purified Neutrophil Chemotactic Factor of Dirofilaria immitis (NCF-Di) and Its Secretion from Adult Worms
Authors: 中西, 弘有 / 堀井, 洋一郎 / 寺島, 賢二郎 / 藤田, 紘一郎
Authors (alternative): Nakanishi, Hirokuni / Horii, Yoichiro / Terashima, Kenjiro / Fujita, Koichiro
Issue Date: 30-Jun-1988
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 30(2). p175-178, 1988
Abstract: 最近,我々は犬糸状虫(Dirofilaria immitis)より好中球遊走活性を持つポリペプチド(NCF-Di)を単離・精製した.本研究ではNCF-Diのin vivoでの作用と,虫体からの放出の有無について調べた. NCF-Diを0.5, 5, 50, 500μg/mlの濃度でモルモット皮内に注射すると,2時間後には注射部位に著明な好中球の浸潤がみられた.その反応は注射後6時間をピークとするもので、浸潤細胞数は濃度に依存して増加した.また,注射部位には好中球以外の細胞はわずかしかみられなかった.この事から,犬糸状虫由来のNCF-Diはモルモット体内で好中球を直接かつ選択的に誘引する事が明らかとなった.また,犬糸状虫成虫を短時間培養すると培養液中に著明な好中球遊走活性が認められることから,この物質(NCF-Di)は虫体から放出(排泄)されているものである事がわかった. / The neutrophil chemotactic factor (NCF-Di) has recently been purified from Dirofilaria immitis as a polypeptide by us. In the present study, in vivo activity of NCF-Di and its secretion from the adult worm of D. immitis were investigated. When various concentrations of NCF-Di (0.5 to 500μg/ml) were injected intradermally into the back of guinea pigs, a large number of neutrophils infiltrated at the injection sites 2 hours after the injection. The severity of the reaction reached the peak at 6 hours and was dose dependent. Whereas only a few other cells infiltrated. It is indicated that the neutrophil chemotactic factor of D. immitis could directly and selectively attract neutrophils in vivo. When intact adult worms of D. immitis were cultured, significant neutrophil chemotactic activity was detected in the culture supernatant of worms. This result suggests possible secretion of the neutrophil chemotactic factor by D. immitis adults in the hosts.
URI: http://hdl.handle.net/10069/4525
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 30, No. 2

Citable URI : http://hdl.handle.net/10069/4525

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