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Optimal Conditions of Plaque Titration of Japanese Encephalitis Virus on BHK21 Cells


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Title: Optimal Conditions of Plaque Titration of Japanese Encephalitis Virus on BHK21 Cells
Authors: Shameem, Golam Masud Mohammad / Morita, Kouichi / Haishi, Shozo / Igarashi, Akira
Issue Date: 27-Dec-1989
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 31(4). p151-159, 1989
Abstract: Optimal conditions for the infectivity titration by plaque formation (PFU) of Japanese encephalitis (JE) virus on mammalian BHK21 cells were investigated. Early virus inoculation at 6 hr after seeding low concentration of the cells (0.25×10^5/ml) produced larger and clearer plaques compared with higher concentration or longer incubation (1-2 days) of the cells. However, the virus titer increased by the incubation time until 2 days to become the highest for this inoculum cell concentration, and up to 1 day for the inoculum cell concentration of 0.5 or 1.0×10^5/ml. While the virus titer did not differ significantly by the time of cell culture for the inoculum cell concentration of 2.0×10^5/ml. Supplementation of 9% fetal bovine serum (FBS) to the cell growth medium was mandatory for clear plaque formation. Among several methyl cellulose (MC) concentrations in the overlay medium, a moderate concentration of 1.25% was optimal to produce clear and distinct plaques on 1 day's culture from 2.0×10^5 cells/ml.
URI: http://hdl.handle.net/10069/4554
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 31, No. 4

Citable URI : http://hdl.handle.net/10069/4554

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