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Immunogenicity of Japanese Encephalitis Virus Envelope Glycoprotein E Prepared by Four Different Methods


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Title: Immunogenicity of Japanese Encephalitis Virus Envelope Glycoprotein E Prepared by Four Different Methods
Authors: Srivastava, Ashok Kumar / Morita, Kouichi / Igarashi, Akira
Issue Date: 28-Sep-1990
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 32(3). p103-113, 1990
Abstract: Envelope glycoprotein (E) of Japanese encephalitis (JE) virus was prepared from purified virion by Triton X-100 (TX-100) treatment (E-TX), polyacrylamide gel electrophoresis (PAGE) in the presence of sodium dodecyl sulphate (SDS) after reduction (E-2ME-SDS) or without reduction (E-IAA-SDS), and high performance liquid chromatography after denaturation with guanidine hydrochloride (E-HPLC). E-2ME-SDS, E-IAA-SDS and particularly E-HPLC were less immunogenic than E-TX, complete virion or formalin-inactivated JE vaccine, which were almost similarly immunogenic to produce anti-JE ELISA and neutralizing (N) antibodies. E-HPLC produced low but definite N antibodies with highest N/ELISA ratio among all immunogens tested. The result indicated that most of the JE virus E protein N and ELISA epitopes were conformational, but some N epitope(s) were resistant to denaturation and could be preserved in E-HPLC better than the ELISA epitopes.
URI: http://hdl.handle.net/10069/4568
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 32, No. 3

Citable URI : http://hdl.handle.net/10069/4568

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