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Gram Stain and Quantitative Sputum Culture in Bacteriological Assessment of Lower Respiratory Tract Infection


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Title: Gram Stain and Quantitative Sputum Culture in Bacteriological Assessment of Lower Respiratory Tract Infection
Authors: Odhiambo, Joseph A. / Oishi, Kazunori / Watanabe, Kiwao / Nagatake, Tsuyoshi / Suzuki, Hiroshi / Matsumoto, Keizo
Issue Date: 28-Sep-1990
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 32(3). p115-120, 1990
Abstract: We evaluated the clinical role of Gram-stained smears and quantitative culture in examining sputum specimens from patients with respiratory infections. Fresh eputum specimens (75 prospectively and 86 retrospectively) from elderly patients with predominantly chronic pulmonary disorders were submitted to this study. The respiratory pathogenic organisms were isolated more frequently from high quality sputum defined by a modified sputum screening system on Gram-stained sputum smears. In this study, 34.8% of specimens examined were qualified for rejection. A significantly positive correlation between the number of dominant bacteria on Gram-stained smears and the subsequently isolated pathogens was demonstrated. The modified sputum screening system used in the present study is recommended for routine laboratory use to save technical and financial resources, and to enhance the diagnostic value of sputum specimens.
URI: http://hdl.handle.net/10069/4569
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 32, No. 3

Citable URI : http://hdl.handle.net/10069/4569

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