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Association of Sindbis Virus Proteins with Vero Cell Cytoskeleton


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Title: Association of Sindbis Virus Proteins with Vero Cell Cytoskeleton
Authors: Ng, Mah Lee / Loy, Boon Pheng / Oei, Coreen
Issue Date: 28-Dec-1990
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 32(4). p155-163, 1990
Abstract: The involvement of Vero cell cytoskeleton in Sindbis virus replication was studied. Cytoskeleton disrupting drugs, i. e. Vinblastine sulphate (Vin) and Cytochalasin B (CB) were used. When the infected cells were exposed to Vin immediately after infection for 9 hours, all virus-specified proteins were still present. The core protein was observed albeit at lower quantity compared with the untreated cells. Treatment with CB did not affect 93K and core proteins but PE2 and E1 were absent in the virus-specified protein profiles. Triton X-100-extracted cells also revealed modification of the remaining cytoskeleton as infection progressed. Generally the filaments became thickened and clumpy from 6 hours post infection (p. i.). Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDSPAGE) anaylsis also revealed the core protein to be affiliated with the remaining cytoskeleton.
URI: http://hdl.handle.net/10069/4574
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 32, No. 4

Citable URI : http://hdl.handle.net/10069/4574

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