DSpace university logo mark
詳細検索
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > 120 熱帯医学研究所 > 120 紀要 > 熱帯医学 > 第33巻 第3号 >

Purification and Characterization of a DNA-Binding Protein of Vibrio cholerae O1


ファイル 記述 サイズフォーマット
tm33_03_05_t.pdf3.06 MBAdobe PDF本文ファイル

タイトル: Purification and Characterization of a DNA-Binding Protein of Vibrio cholerae O1
著者: Ehara, Masahiko / Ichinose, Yoshio / Iwami, Mamoru / Utsunomiya, Akiyoshi / Shimotori, Shoichi
発行日: 1991年 9月30日
出版者: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
引用: 熱帯医学 Tropical medicine 33(3). p67-82, 1991
抄録: A DNA-Binding protein was purified from the cell-surface of Vibrio cholerae O1 by Cellulofine Sulfate affinity chromatography and reversed-phase HPLC. The DNA-binding protein of V. cholerae O1 showed a molecular weight of 9 kDa estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Treatment of the protein with cold acetone and 6 M guanidine-HCl produced little change in the DNA-binding activity of the protein. The binding of the protein to supercoiled plasmid DNA as well as to double-stranded short chromosomal DNA fragments has been demonstrated by gel retardation assay and by labeling of the transblotted protein with non-radioactive DNA. N-terminal amino acid sequence of the protein showed a high degree of homology with that of HU 2 (Laine et al., 1978), NS2 (Mende et al., 1978) from Escherichia coli. Indirect immunocytochemical labeling of ultrathin sections of Lowicryl-embedded V. cholerae O1 cells with the antibody against the protein revealed that the protein associates with bulk DNA and also lies at the cell-surface.
URI: http://hdl.handle.net/10069/4584
ISSN: 03855643
資料タイプ: Departmental Bulletin Paper
出現コレクション:第33巻 第3号

引用URI : http://hdl.handle.net/10069/4584

このリポジトリに保管されている文献はすべて著作権により保護されています。
印刷やダウンロード等データの複製は、調査研究・教育または学習を目的とする場合に限定されます。

 

Valid XHTML 1.0! Copyright © 2006-2015 長崎大学附属図書館 - お問い合わせ Powerd by DSpace