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A Rapid Plaque Assay for Japanese Encephalitis Virus Infectivity by the Presence of Actinomycin D in Overlay Medium


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Title: A Rapid Plaque Assay for Japanese Encephalitis Virus Infectivity by the Presence of Actinomycin D in Overlay Medium
Authors: Daji, Hu / Morita, Kouichi / Igarashi, Akira
Issue Date: 30-Jun-1992
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 34(2). p55-62, 1992
Abstract: An improved rapid plaque assay for Japanese encephalitis (JE) virus infectivity was developed. When Actinomycin D was introduced at 0.2μg/ml concentration into the overlay medium which contained 2% fetal calf serum and 1% methyl cellulose in Eagle's medium, clear plaques of Nakayama strain JE virus were formed 52hr postinfection on BHK21 cells. The improved method provided reasonably rapid result of virus infectivity with less labor and cost than conventional plaque or focus assay.
URI: http://hdl.handle.net/10069/4596
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 34, No. 2

Citable URI : http://hdl.handle.net/10069/4596

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