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Pretreatment of Test Sera with Three Kinds of IgG-Adsorbents Did Not Improve Sensitivity in the Indirect ELISA to Detect Anti-Dengue IgM Antibodies


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Title: Pretreatment of Test Sera with Three Kinds of IgG-Adsorbents Did Not Improve Sensitivity in the Indirect ELISA to Detect Anti-Dengue IgM Antibodies
Authors: Kheong, Chew Tong / Balasubramaniam, Vijayamalar / Sinniah, Mangalam / Igarashi, Akira
Issue Date: 20-Oct-1993
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 35(2). p65-73, 1993
Abstract: Sera from clinical dengue cases in Malaysia were examined to detect IgG- as well as IgM-class anti-dengue antibodies by the indirect ELISA with or without treatment by 3 kinds of IgG-adsorbents. Relative adsorption factor (AF) as determined by the decreased ELISA-OD ratio was 0.42-0.58 for IgG and 0.33-0.39 for IgM, respectively. After IgG-adsorbents treatment, however, the number of anti-dengue IgM-positive serum specimen decreased from 11-20% of the untreated control. The result indicated that pretreatment of test sera with IgG-adsorbents did not improve or even reduced the sensitivity to detect anti-dengue IgM antibodies in the test sera.
URI: http://hdl.handle.net/10069/4624
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 35, No. 2

Citable URI : http://hdl.handle.net/10069/4624

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