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Application of Reverse Transcriptase-Polymerase Chain Reaction for Serotype Determination of Dengue Virus Strains Isolated in North Vietnam, 1991 to 1993


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Title: Application of Reverse Transcriptase-Polymerase Chain Reaction for Serotype Determination of Dengue Virus Strains Isolated in North Vietnam, 1991 to 1993
Authors: Diet, Le Van / Ninh, Truong Nguyen / Lien, Huynh Phuong / Nguyen, Hoang Thuy / Morita, Kouichi / Igarashi, Akira
Issue Date: 28-Dec-1994
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 36(3). p111-115, 1994
Abstract: Reverse transcriptase-polymerase chain reaction (RT-PCR) was applied to determine serotypes of 20 dengue virus strains isolated in north Vietnam in the years of 1991 and 1993. There were 10 strains of dengue type 1 (D1), 9 strains of dengue type 2 (D2) and a single strain of dengue type 4 (D4), respectively. The results completely agreed with the serotypes determined by the immunofluorescent staining (IFA) of the infected cells using dengue type-specific monoclonal antibodies.
URI: http://hdl.handle.net/10069/4679
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 36, No. 3

Citable URI : http://hdl.handle.net/10069/4679

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