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Serotype Identification of Dengue Virus Isolates by an Enzyme-linked Immunosorbent Assay Employing a Protein Denaturing Agent.


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タイトル: Serotype Identification of Dengue Virus Isolates by an Enzyme-linked Immunosorbent Assay Employing a Protein Denaturing Agent.
著者: Nawa, Masaru
発行日: 1995年 3月31日
出版者: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
引用: 熱帯医学 Tropical medicine 36(4). p140-147, 1995
抄録: Serotypes of dengue isolates were identified successfully by an enzyme-linked immunosorbent assay (ELISA) reacting with crude viral antigens and conventional hyperimmunized anti-dengue mouse antibodies. In the ELISA, a protein denaturing agent, sodium thiocyanate (NaSCN) was employed to discriminate between serotype specific reaction and nonspecific cross-reaction among four serotypes of the virus. Antigen fractions in sucrose gradient were highly cross-reactive to both homologous and heterologous antibodies in the absence of NaSCN in the ELISA, and their serotype specificities were improved by the treatment of NaSCN in the ELISA. Fifteen out of 38 strains (ca. 38%) and 12 out of 38 strains (ca. 31.5%) of dengue isolates were recovered and identified by IFA staining and the ELISA from field specimens kept at -80℃ for 7 years, respectively.
URI: http://hdl.handle.net/10069/4684
ISSN: 03855643
資料タイプ: Departmental Bulletin Paper
出現コレクション:第36巻 第4号

引用URI : http://hdl.handle.net/10069/4684

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