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Comparison of Two Different Methods for the Purification of Polymerase Chain Reaction (PCR) Products Used in Direct Sequencing, in an Applied Biosystems 373A DNA Sequencing System


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Title: Comparison of Two Different Methods for the Purification of Polymerase Chain Reaction (PCR) Products Used in Direct Sequencing, in an Applied Biosystems 373A DNA Sequencing System
Authors: Thayan, Ravindran / Morita, Kouichi / Vijayamalar, Balasubramaniam / Chew, Tong Keong / Sinniah, Mangalam / Igarashi, Akira
Issue Date: 23-Jun-1995
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 37(1). p29-33, 1995
Abstract: This study was carried out to compare the efficiency of two different purification methods used for removing excess primers and short oligonucleotides from PCR products. Our aim was to sequence PCR product directly. The two different purification methods were micro filter spin unit system (Millipore) and Quick Spin Column (Boehringer Mannheim Biochemica). The results indicated that the purification method using microfilter spin unit system was better and yielded better suquencing pattern compared to quick Spin Column.
URI: http://hdl.handle.net/10069/4701
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 37, No. 1

Citable URI : http://hdl.handle.net/10069/4701

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