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Production of Type 2 Dengue (D2) Monoclonal Antibody and Cell Culture Derived D2 Antigen for Use in Dengue IgM-Capture ELISA


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Title: Production of Type 2 Dengue (D2) Monoclonal Antibody and Cell Culture Derived D2 Antigen for Use in Dengue IgM-Capture ELISA
Authors: Igarashi, Akira / Mohamed, Haliman / Yusof, Apandi / Sinniah, Mangalam / Tanaka, Hiroshi
Issue Date: 29-Mar-1996
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 37(4). p165-173, 1996
Abstract: In order to strengthen laboratory diagnostic capability on dengue by IgM-ELISA in Malaysia, type 2 dengue (D2) monoclonal antibody (MAb) and cell culture-derived D2 antigen were prepared as culture fluid of hybridoma cell line and D2-infected C6/36 culture fluid, respectively. Comparative IgM-ELISA was carried out on 90 serum specimens from dengue patients using newly prepared reagents in comparison with routine reagents (D2-MAb mouse ascitic fluid from CDC, USA, and D2 antigen extracted from infected suckling mouse brains). The newly prepared MAb at 2-10 fold higher concentration than that estimated by antigen detection ELISA, in combination with the undiluted D2-infected C6/36 cell culture fluid, provided comparable sensitivity (93.3-97.9%) to that of routine reagents (90.0-98.5%).
URI: http://hdl.handle.net/10069/4717
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 37, No. 4

Citable URI : http://hdl.handle.net/10069/4717

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