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Immunopathological Characterization of liposome Adjuvant Coated with Mannan


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Title: Immunopathological Characterization of liposome Adjuvant Coated with Mannan
Authors: Skikata, Kohdo / Ohishi, Kazue / Kawai, Hiromi / Fukasawa, Masashi / Sakakibara, Ryuzo / Ishiguro, Masatsune / Itakura, Hideyo / Hatanaka, Masakazu / Sugimoto, Masanobu
Issue Date: 24-Mar-1997
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 38(3/4). p91-99, 1997
Abstract: The adjuvant activity of liposomes coated with mannan-cholesterol was studied in mice. Ovalbumin (OVA) was reconstituted into liposomes as a model antigen. The adjuvant activity was assessed by the following two immunological responses: delayed-type hypersensitivity (DTH) footpad swelling responses and in vitro release of interferon-γ and interleukin-4 by regional lymph node cells. First, we studied dose effects on DTH responses of total lipid, mannan-cholesterol and OVA used for liposomes. The minimal doses per mouse required for the induction of optimal responses were as follows; 1μg of OVA, 10μg of mannan-cholesterol and 336μg of total lipid. Second, immunological and histopathological studies showed the following two points: 1) mannan-coated liposomes induced a tuberculintype DTH response while non-coated liposomes elicited a Jones-Mote reaction, and 2) mannan-coated liposomes induced obvious microabscesses but non-coated liposome did not. Third, the inoculation of mannan-coated liposomes rendered the regional lymph node cells to release a large amount of interferon-γ with little IL-4 against OVA while non-coated lipo-some released neither of the lymphokines. These results indicated that mannan-coated lipo-somes are a potent adjuvant to induce type 1 helper T cells but have a disadvantage to form microabscesses at the inoculation sites.
URI: http://hdl.handle.net/10069/4730
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 38, No. 3/4

Citable URI : http://hdl.handle.net/10069/4730

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