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Dengue IgM-capture ELISA Using Heat-inactivated and Unheated Control Antigens Prepared as Infected C6/36 Cell Culture Fluid


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Title: Dengue IgM-capture ELISA Using Heat-inactivated and Unheated Control Antigens Prepared as Infected C6/36 Cell Culture Fluid
Authors: Kanashiro, Rosa S. / Igarashi, Akira
Issue Date: 29-Jan-1999
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 40(3). p149-156, 1999
Abstract: A panel of 91 dengue patient's sera were examined in the IgM-capture ELISA using each of the 4 types of dengue antigens prepared as infected C6/36 cell culture fluid, with or without brief heat inactivation at 56℃. The result did not show any significant difference between the results obtained by both antigens. Therefore, dengue antigens for serodiagnosis by the IgM-capture ELISA could be prepared as infected C6/36 cell culture fluid with brief heat-inactivation for biosafety requirement.
URI: http://hdl.handle.net/10069/4757
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 40, No. 3

Citable URI : http://hdl.handle.net/10069/4757

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