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酸素活性試験を応用したワムシの大量培養診断


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タイトル: 酸素活性試験を応用したワムシの大量培養診断
著者: アドリアナ, ベレン, デ, アラウジョ / 萩原, 篤志
著者(別表記) : Adriana, Belem De Araujo / Hagiwara, Atsushi
発行日: 2001年 3月
出版者: 長崎大学水産学部
引用: 長崎大学水産学部研究報告 v.82 p.85-91 , 2001
抄録: Early detection of stress in rotifer mass cultures is important for aquaculturists because it allows preventative action to be taken. We applied measurement of rotifer enzyme activity for evaluating the status of rotifer mass cultures in 1 kl tank at 28oC. Two tests were conducted; one was to directly measure the enzyme activity of mass cultured rotifers, and the other was to monitor the enzyme activity of neonates from resting eggs after exposing them to a filtrate of rotifer mass culture water. From trials using three substrates (cFDAam, PLA2, FDGlu) against neonates from resting eggs, esterase (cFDAam as a substrate) activity increased one day prior to the decline of rotifer population growth, indicating that this enzyme can be used as a tool for early warning for detection of instability of the mass culture practices conducted at a hatchery in Nagasaki. The daily change of rotifer population density was most parallel to that of glucosidase activity. When direct measurement of enzyme activity was conducted for mass cultured rotifers, however, esterase activity did not reveal a relationship with rotifer population growth. It was suggested that the enzyme activity test is appropriate when used for rotifers with the same size and age.
URI: http://hdl.handle.net/10069/6540
ISSN: 05471427
資料タイプ: Departmental Bulletin Paper
原稿種類: publisher
出現コレクション:第82号

引用URI : http://hdl.handle.net/10069/6540

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