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Evaluation of Newly Developed Oxygen Meters with Multi-Channels and Disposable Oxygen Electrode Sensors for Antimicrobial Susceptibility Testing


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Title: Evaluation of Newly Developed Oxygen Meters with Multi-Channels and Disposable Oxygen Electrode Sensors for Antimicrobial Susceptibility Testing
Authors: Kitahara, Takashi / Koyama, Nao / Matsuda, Junichi / Hirakata, Yoichi / Kamihira, Shimeru / Kohno, Shigeru / Nakashima, Mikiro / Sasaki, Hitoshi
Issue Date: Sep-2003
Publisher: Pharmaceutical Society of Japan
Citation: Biological & Pharmaceutical Bulletin v.26(9) p.1229-1234, 2003
Abstract: The objective of this study was to investigate the applicability of new oxygen meters with multi-channels and disposable oxygen electrode sensors (DOX-96) on the antimicrobial susceptibility testing of clinical bacterial isolates. The oxygen amount in the wells of 96-well plates was converted into current through electrodes. Bacterial inoculation decreased the oxygen amount in the wells because viable bacteria consume the oxygen. On the other hand, a failure of bacteria to consume oxygen was observed in the presence of potent antimicrobial agents, representing a serious arrest of bacterial metabolism usually leading to stasis or death. Based on these results, the minimum inhibitory concentration was determined by DOX-96 (MICdox). The MICdox showed good agreement with MIC measured by the standard broth microdilution method (98.2%). DOX-96 was also useful for turbid samples such as Mueller-Hinton broth containing 0.1% lipid emulsion. The MICdox in turbid samples showed good agreement with those in clear samples (100.0%). These results indicate that the newly developed DOX-96 is very useful in antimicrobial susceptibility testing even in turbid clinical samples such as colloidal products and turbid biological
Keywords: oxygen electrode / antimicrobial susceptibility testing / oxygen meter / minimum inhibitory concentration / lipid emulsion
URI: http://hdl.handle.net/10069/8342
ISSN: 09186158
DOI: 10.1248/bpb.26.1229
PubMed ID: 12951463
Relational Links: http://dx.doi.org/10.1248/bpb.26.1229
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/8342

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