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Transport of Acebutolol through Rabbit Corneal Epithelium

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タイトル: Transport of Acebutolol through Rabbit Corneal Epithelium
著者: Kawazu, Kouichi / Oshita, Akemi / Nakamura, Tadahiro / Nakashima, Mikiro / Ichikawa, Nobuhiro / Sasaki, Hitoshi
発行日: 2006年 4月
出版者: Pharmaceutical Society of Japan
引用: Biological & Pharmaceutical Bulletin v.29(4) p.846-849, 2006
抄録: The purpose of this study is to characterize transport of acebutolol through the corneal epithelium. Cultured normal rabbit corneal epithelial cells (RCEC) were used to investigate the drug transport. Primary RCEC were seeded on a filter membrane of Transwell-COL? insert coated with fibronectin and were grown in Dulbecco's modified Eagle's medium/nutrient mixture F-12 with various supplements. Measurements of acebutolol permeability through RCEC layer were carried out to assess transcellular permeability coefficient (Ptranscell) in the absence or presence of inhibitors. Paracellular permeability coefficient (Pparacell) was calculated by permeability coefficient of hydrophilic drugs (Pcell). The transcellular permeability of acebutolol from apical side to basal side (A-to-B) showed concentration-dependency. The acebutolol flux in the A-to-B direction was smaller than that of opposite direction. Sodium azide, verapamil, and cyclosporin A enhanced the transcellular permeability of acebutolol in the A-to-B direction. Acebutolol permeability through an excised rabbit cornea was also increased by verapamil. Thus, it was suggested that acebutolol was actively secreted via P-glycoprotein in a corneal epithelium.
キーワード: cultured rabbit cornea / acebutolol / transporter / P-glycoprotein / corneal epithelium
URI: http://hdl.handle.net/10069/8344
ISSN: 09186158
DOI: 10.1248/bpb.29.846
PubMed ID: 16595934
関連リンク : http://dx.doi.org/10.1248/bpb.29.846
資料タイプ: Journal Article
原稿種類: publisher
出現コレクション:130 学術雑誌論文

引用URI : http://hdl.handle.net/10069/8344



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