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A Multi-Generation Sublethal Assay of Phenols Using the Nematode Caenorhabditis elegans


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Title: A Multi-Generation Sublethal Assay of Phenols Using the Nematode Caenorhabditis elegans
Authors: Tominaga, Nobuaki / Kohra, Shinya / Iguchi, Taisen / Arizono, Koji
Issue Date: Dec-2003
Publisher: Pharmaceutical Society of Japan
Citation: Journal of Health Science v.49(6) p.459-463, 2003
Abstract: The nematode Caenorhabditis elegans (C. elegans) was used for a multiple-generation toxicity bioassay of phenols. We examined the sublethal toxicity (fecundity and reproduction) of bisphenol A (BisA), 4-(1-adamantyl) phenol (Adp), and 4,4′-(1,3-adamantanediyl) diphenol (AdDP) over five generations using a Nematode Growth Medium (NGM) 1.7% agar plate. In the fourth generation, the phenols affected the fecundity rate of C. elegans at doses 100- to 10000-fold lower than the LC50s. In particular, at 1 nM BisA, the number of worms decreased significantly to about 50% of control (p < 0.05). A comparison of the number of viable worms and eggs suggested that the phenols exert hatching toxicity. In addition, individuals with an abnormality in the vulva, which could not ovulate, appeared on the phenol-containing plates. We confirmed that phenols disrupt reproduction in C. elegans and that the method using NGM agar plates facilitates multi-generational toxicity tests of chemicals that are poorly soluble in water.
Keywords: Caenorhabditis elegans / reproduction / fecundity / reproductive disruption
URI: http://hdl.handle.net/10069/8372
ISSN: 13449702
DOI: 10.1248/jhs.49.459
Relational Links: http://dx.doi.org/10.1248/jhs.49.459
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/8372

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