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A Multi-Generation Sublethal Assay of Phenols Using the Nematode Caenorhabditis elegans

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タイトル: A Multi-Generation Sublethal Assay of Phenols Using the Nematode Caenorhabditis elegans
著者: Tominaga, Nobuaki / Kohra, Shinya / Iguchi, Taisen / Arizono, Koji
発行日: 2003年12月
出版者: Pharmaceutical Society of Japan
引用: Journal of Health Science v.49(6) p.459-463, 2003
抄録: The nematode Caenorhabditis elegans (C. elegans) was used for a multiple-generation toxicity bioassay of phenols. We examined the sublethal toxicity (fecundity and reproduction) of bisphenol A (BisA), 4-(1-adamantyl) phenol (Adp), and 4,4′-(1,3-adamantanediyl) diphenol (AdDP) over five generations using a Nematode Growth Medium (NGM) 1.7% agar plate. In the fourth generation, the phenols affected the fecundity rate of C. elegans at doses 100- to 10000-fold lower than the LC50s. In particular, at 1 nM BisA, the number of worms decreased significantly to about 50% of control (p < 0.05). A comparison of the number of viable worms and eggs suggested that the phenols exert hatching toxicity. In addition, individuals with an abnormality in the vulva, which could not ovulate, appeared on the phenol-containing plates. We confirmed that phenols disrupt reproduction in C. elegans and that the method using NGM agar plates facilitates multi-generational toxicity tests of chemicals that are poorly soluble in water.
キーワード: Caenorhabditis elegans / reproduction / fecundity / reproductive disruption
URI: http://hdl.handle.net/10069/8372
ISSN: 13449702
DOI: 10.1248/jhs.49.459
関連リンク : http://dx.doi.org/10.1248/jhs.49.459
資料タイプ: Journal Article
原稿種類: publisher
出現コレクション:070 学術雑誌論文

引用URI : http://hdl.handle.net/10069/8372



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