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Unilateral Kidney-Selective Gene Transfer Following the Administration of Naked Plasmid DNA to the Kidney Surface in Mice


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Title: Unilateral Kidney-Selective Gene Transfer Following the Administration of Naked Plasmid DNA to the Kidney Surface in Mice
Authors: Hirayama, Ryu / Nishida, Koyo / Fumoto, Shintaro / Nakashima, Mikiro / Sasaki, Hitoshi / Nakamura, Junzo
Issue Date: Jan-2005
Publisher: Pharmaceutical Society of Japan
Citation: Biological & Pharmaceutical Bulletin v.28(1) p.181-184, 2005
Abstract: We developed a gene transfer following the administration of naked plasmid DNA (pDNA) to the kidney surface in mice, and found that the luciferase levels produced in the applied kidney were significantly higher than those produced in another kidney. In contrast, stable renal gene expression was not observed in the case of intraperitoneal or intravenous administration of pDNA. The level of gene expression after instillation of pDNA to the kidney surface reached maximum at 12 h and gradually diminished thereafter. The production of luciferase was saturated at 5 μg of pDNA, and was not affected by instillation volume. Furthermore, pDNA uptake from the kidney surface was proved by in situ experiments using a glass-made diffusion cell. We demonstrated a novel unilateral kidney-selective gene transfer following the administration of naked pDNA to the kidney surface in mice.
Keywords: gene therapy / plasmid DNA / kidney / gene transfer / luciferase / mice
URI: http://hdl.handle.net/10069/8392
ISSN: 09186158
DOI: 10.1248/bpb.28.181
PubMed ID: 15635189
Relational Links: http://dx.doi.org/10.1248/bpb.28.181
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/8392

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