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Unilateral Kidney-Selective Gene Transfer Following the Administration of Naked Plasmid DNA to the Kidney Surface in Mice


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タイトル: Unilateral Kidney-Selective Gene Transfer Following the Administration of Naked Plasmid DNA to the Kidney Surface in Mice
著者: Hirayama, Ryu / Nishida, Koyo / Fumoto, Shintaro / Nakashima, Mikiro / Sasaki, Hitoshi / Nakamura, Junzo
発行日: 2005年 1月
出版者: Pharmaceutical Society of Japan
引用: Biological & Pharmaceutical Bulletin v.28(1) p.181-184, 2005
抄録: We developed a gene transfer following the administration of naked plasmid DNA (pDNA) to the kidney surface in mice, and found that the luciferase levels produced in the applied kidney were significantly higher than those produced in another kidney. In contrast, stable renal gene expression was not observed in the case of intraperitoneal or intravenous administration of pDNA. The level of gene expression after instillation of pDNA to the kidney surface reached maximum at 12 h and gradually diminished thereafter. The production of luciferase was saturated at 5 μg of pDNA, and was not affected by instillation volume. Furthermore, pDNA uptake from the kidney surface was proved by in situ experiments using a glass-made diffusion cell. We demonstrated a novel unilateral kidney-selective gene transfer following the administration of naked pDNA to the kidney surface in mice.
キーワード: gene therapy / plasmid DNA / kidney / gene transfer / luciferase / mice
URI: http://hdl.handle.net/10069/8392
ISSN: 09186158
DOI: 10.1248/bpb.28.181
PubMed ID: 15635189
関連リンク : http://dx.doi.org/10.1248/bpb.28.181
資料タイプ: Journal Article
原稿種類: publisher
出現コレクション:050 学術雑誌論文

引用URI : http://hdl.handle.net/10069/8392

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