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Stomach-Selective Gene Transfer Following the Administration of Naked Plasmid DNA onto the Gastric Serosal Surface in Mice


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Title: Stomach-Selective Gene Transfer Following the Administration of Naked Plasmid DNA onto the Gastric Serosal Surface in Mice
Authors: Nakamura, Junzo / Fumoto, Shintaro / Shoji, Keiko / Kodama, Yukinobu / Nishi, Junya / Nakashima, Mikiro / Sasaki, Hitoshi / Nishida, Koyo
Issue Date: Oct-2006
Publisher: Pharmaceutical Society of Japan
Citation: Biological & Pharmaceutical Bulletin v.29(10) p.2082-2086, 2006
Abstract: The purpose of the present study was to achieve a stomach-selective gene transfer following the administration of naked plasmid DNA (pDNA) onto the gastric serosal surface in mice. Gene expression in the stomach and other tissues was evaluated by firefly luciferase activity. Six hours after gastric serosal surface instillation of naked pDNA, high gene expression in the stomach was observed. On the contrary, intravenous and intraperitoneal injection of naked pDNA exhibited no detectable gene expression. Following instillation of naked pDNA onto the gastric serosal surface, gene expression in the stomach was significantly higher than in other tissues. Gene expression in the stomach was highest 12 h after the instillation and thereafter decreased gradually. Utilizing a glass-made diffusion cell that is able to limit the contact dimension between the gastric serosal surface and the naked pDNA solution administered, site-specific gene expression in the stomach was achieved. This novel gene transfer method is expected to be a safe and effective treatment against serious stomach diseases.
Keywords: gene therapy / plasmid DNA / stomach / transfection / mouse / luciferase
URI: http://hdl.handle.net/10069/8394
ISSN: 09186158
DOI: 10.1248/bpb.29.2082
PubMed ID: 17015955
Relational Links: http://dx.doi.org/10.1248/bpb.29.2082
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/8394

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