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Surface Plasmon Resonance Analysis for the Screening of Anti-prion Compounds

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Title: Surface Plasmon Resonance Analysis for the Screening of Anti-prion Compounds
Authors: Kawatake, Satoshi / Nishimura, Yuki / Sakaguchi, Suehiro / Iwaki, Toru / Doh-Ura, Katsumi
Issue Date: May-2006
Publisher: Pharmaceutical Society of Japan
Citation: Biological & Pharmaceutical Bulletin v.29(5) p.927-932, 2006
Abstract: The interaction of anti-prion compounds and amyloid binding dyes with a carboxy-terminal domain of prion protein (PrP121?231) was examined using surface plasmon resonance (SPR) and compared with inhibition activities of abnormal PrP formation in scrapie-infected cells. Most examined compounds had affinities for PrP121?231: antimalarials had low affinities, whereas Congo red, phthalocyanine and thioflavin S had high affinities. The SPR binding response correlated with the inhibition activity of abnormal PrP formation. Several drugs were screened using SPR to verify the findings: propranolol was identified as a new anti-prion compound. This fact indicates that drug screenings by this assay are useful.
Keywords: anti-prion compound / surface plasmon resonance / scrapie-infected cell / screening / recombinant prion protein
URI: http://hdl.handle.net/10069/8397
ISSN: 09186158
DOI: 10.1248/bpb.29.927
PubMed ID: 16651721
Relational Links: http://dx.doi.org/10.1248/bpb.29.927
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/8397

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