DSpace university logo mark
Advanced Search
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > School of Medicine > Bulletin > Acta Medica Nagasakiensia > Volume 50, No. supl.1 >

T-cell Receptor Assay and Reticulocyte-Micronuclei Assay as Biological Dosimeters for Ionizing Radiation in Humans

File Description SizeFormat
acta50_s1_15.pdf919.43 kBAdobe PDFView/Open

Title: T-cell Receptor Assay and Reticulocyte-Micronuclei Assay as Biological Dosimeters for Ionizing Radiation in Humans
Authors: Vershenya, Stanislav / Biko, Johannes / Lorenz, Reinhard / Reiners, Christoph / Stopper, Helga / Grawe, Jan / Hempel, Klaus
Issue Date: Dec-2005
Citation: Acta medica Nagasakiensia. 2005, 50(supl.1), p.15-21
Abstract: In radiation accidents, biological methods are used for dosimetry if the radiation dose could not be measured by physical means. The knowledge of individual dose is a prerequisite for planning medical treatment and for health risk evaluations. In this paper we represent the summary of biodosimetrical methods used in our laboratory in the patients treated with radioiodine for thyroid cancer. The dose-response relationship was measured by the flow cytometry-based micronucleus assay in transferrin receptor positive reticulocytes (MN-Tf-Ret assay) and by the T-cell receptor (TCR) assay in CD4 lymphocytes. Compared to our previous works, the database for TCR assay was significantly improved, and two groups of thyroid cancer patients (from Belarus and Germany) were studied. In the MN-Tf-Ret test, mutant frequency increased proportionally with the radiation dose, whereas in the TCR-test the dose relationship could be described by an exponential equation which takes into account the limited lifespan of TCR mutants. Finally, these assays are compared with other biodosimetrical assays used in patients treated with radioiodine.
Keywords: T-cellreceptor / Micronucleus / CD71positivereticulocyte / Transferrinpositivereticulocyte / Mutation / Radiation
URI: http://hdl.handle.net/10069/9306
ISSN: 00016055
Relational Links: http://joi.jlc.jst.go.jp/JST.JSTAGE/amn/50.S15
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 50, No. supl.1

Citable URI : http://hdl.handle.net/10069/9306

All items in NAOSITE are protected by copyright, with all rights reserved.


Valid XHTML 1.0! Copyright © 2006-2015 Nagasaki University Library - Feedback Powerd by DSpace