DSpace university logo mark
Advanced Search
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > School of Medicine > Bulletin > Acta Medica Nagasakiensia > Volume 50, No. supl.1 >

Evidence for Induction of DNA Double Strand Breaks in the Bystander Response to Targeted Soft X-Rays in Repair Deficient CHO Cells


File Description SizeFormat
acta50_s1_69.pdf428.24 kBAdobe PDFView/Open

Title: Evidence for Induction of DNA Double Strand Breaks in the Bystander Response to Targeted Soft X-Rays in Repair Deficient CHO Cells
Authors: Kashino, Genro / Suzuki, Keiji / M.Prise, Kevin / Schettino, Giuseppe / Folkard, Melvyn / Vojnovic, Borivoj / D.Michael, Barry / Kodama, Seiji / Matsuda, Naoki / Watanabe, Masami
Issue Date: Dec-2005
Citation: Acta medica Nagasakiensia. 2005, 50(supl.1), p.69-71
Abstract: Evidence is accumulating that irradiated cells produce some signals which interact with non-exposed cells in the same population. Here, we analysed the mechanism of such a bystander effect from targeted cells to non-targeted cells. Firstly, in order to investigate the bystander effect in CHO cell lines we irradiated a single cell within a population and scored the formation of micronuclei. When a single nucleus in the population, of double strand break repair deficient xrs5 cells, was targeted with 1Gy of Al-K soft X-rays, elevated numbers of micronuclei were induced in the neighbouring unirradiated cells. The induction of micronuclei was also observed when conditioned medium was transferred from irradiated to non-irradiated xrs5 cells. These results suggest that DNA double strand breaks are caused by factors secreted in the medium from irradiated cells. To clarify the involvements of radical species in the bystander response, cells were treated with 0.5%DMSO 1 hour before irradiation and then bystander effects were estimated in xrs5 cells. The results showed clearly that DMSO treatment during X-irradiation suppress the induction of micronuclei in bystander xrs5 cells, when conditioned medium was transferred from irradiated xrs5 cells. Therefore, it is suggested that radical species induced by ionizing radiation are important for producing bystander signals.
Keywords: Bystandereffect / DNArepair / DNAdoublestrandbreak / Basedamage / SoftX-ray / Microbeam
URI: http://hdl.handle.net/10069/9315
ISSN: 00016055
Relational Links: http://joi.jlc.jst.go.jp/JST.JSTAGE/amn/50.S69
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 50, No. supl.1

Citable URI : http://hdl.handle.net/10069/9315

All items in NAOSITE are protected by copyright, with all rights reserved.

 

Valid XHTML 1.0! Copyright © 2006-2015 Nagasaki University Library - Feedback Powerd by DSpace