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Evidence for Induction of DNA Double Strand Breaks in the Bystander Response to Targeted Soft X-Rays in Repair Deficient CHO Cells


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タイトル: Evidence for Induction of DNA Double Strand Breaks in the Bystander Response to Targeted Soft X-Rays in Repair Deficient CHO Cells
著者: Kashino, Genro / Suzuki, Keiji / M.Prise, Kevin / Schettino, Giuseppe / Folkard, Melvyn / Vojnovic, Borivoj / D.Michael, Barry / Kodama, Seiji / Matsuda, Naoki / Watanabe, Masami
発行日: 2005年12月
引用: Acta medica Nagasakiensia. 2005, 50(supl.1), p.69-71
抄録: Evidence is accumulating that irradiated cells produce some signals which interact with non-exposed cells in the same population. Here, we analysed the mechanism of such a bystander effect from targeted cells to non-targeted cells. Firstly, in order to investigate the bystander effect in CHO cell lines we irradiated a single cell within a population and scored the formation of micronuclei. When a single nucleus in the population, of double strand break repair deficient xrs5 cells, was targeted with 1Gy of Al-K soft X-rays, elevated numbers of micronuclei were induced in the neighbouring unirradiated cells. The induction of micronuclei was also observed when conditioned medium was transferred from irradiated to non-irradiated xrs5 cells. These results suggest that DNA double strand breaks are caused by factors secreted in the medium from irradiated cells. To clarify the involvements of radical species in the bystander response, cells were treated with 0.5%DMSO 1 hour before irradiation and then bystander effects were estimated in xrs5 cells. The results showed clearly that DMSO treatment during X-irradiation suppress the induction of micronuclei in bystander xrs5 cells, when conditioned medium was transferred from irradiated xrs5 cells. Therefore, it is suggested that radical species induced by ionizing radiation are important for producing bystander signals.
キーワード: Bystandereffect / DNArepair / DNAdoublestrandbreak / Basedamage / SoftX-ray / Microbeam
URI: http://hdl.handle.net/10069/9315
ISSN: 00016055
関連リンク : http://joi.jlc.jst.go.jp/JST.JSTAGE/amn/50.S69
資料タイプ: Departmental Bulletin Paper
原稿種類: publisher
出現コレクション:Volume 50, No. supl.1

引用URI : http://hdl.handle.net/10069/9315

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