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Anti-Macrophage Migration Inhibitory Factor Antibody Suppresses Chronic Rejection of Heterotopically Transplanted Trachea in Rats


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Title: Anti-Macrophage Migration Inhibitory Factor Antibody Suppresses Chronic Rejection of Heterotopically Transplanted Trachea in Rats
Authors: Sumida, Yorihisa / Yamashita, Hideki / Inoue, Masao / Ohsawa, Kazutaka / Sato, Hiroshi / Nanashima, Atsushi / Nagayasu, Takeshi
Issue Date: Jun-2006
Citation: Acta medica Nagasakiensia. 2006, 51(2), p.51-56
Abstract: Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine essential for delayed hypersensitivity in vivo, and is involved in bronchiolitis obliterans and late rejection in lung transplantation. We tested here whether neutralization of MIF using anti-MIF antibody prevents such a response. We examined the MIF mRNA expression level and changes in allograft tracheal epithelium and intraluminal obstruction in a rat allograft model. Lewis rat (RT1l) underwent heterotopic tracheal transplantation from Brown Norway rats (RT1n) in the omentum. Anti-MIF antibody was injected in the peritoneum. Rats were divided into three groups (non-treated allograft, allograft treated with normal rabbit IgG and allograft treated with anti-MIF antibody). Implants were harvested on days 7 or 21 for histological analysis. MIF mRNA expression was higher in the allograft at days 7 and 21 than in the isograft. The epithelium in non-treated allograft was almost absent at day 7. The epithelial height in the anti-MIF-treated graft was higher than that in normal IgG-treated grafts. The intraluminal space was mostly replaced by granulation tissue at day 21 in the untreated group. The proportion of obliterans was lowest in the anti-MIF group, the second lowest in the untreated group and the third lowest in the normal IgG-treated grafts, and the difference was significant (p<0.001) between the first two groups. Our results indicate that anti-MIF antibody suppresses allogenic tracheal rejection.
Keywords: Lungtransplantation / Trachea / Macrophagemigrationinhibitoryfactor / Allograft / Chronicrejection / Bronchiolitisobliterans
URI: http://hdl.handle.net/10069/9360
ISSN: 00016055
Relational Links: http://joi.jlc.jst.go.jp/JST.JSTAGE/amn/51.51
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 51, No. 2

Citable URI : http://hdl.handle.net/10069/9360

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