DSpace university logo mark
Advanced Search
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > Lectures and Symposiums > Nagasaki Symposium on Nano-Dynamics 2008 (NSND2008) >

Characterization of Domain 3 and Its α-Helical Region of the Hemolytic Lectin CEL-III Expressed as Glutathione S-Transferase-Fusion Proteins


File Description SizeFormat
NSND2008_P14.pdf455.79 kBAdobe PDFView/Open

Title: Characterization of Domain 3 and Its α-Helical Region of the Hemolytic Lectin CEL-III Expressed as Glutathione S-Transferase-Fusion Proteins
Authors: Hisamatau, Keigo / Unno, Hideaki / Goda, Shuichiro / Hatakeyama, Tomomitsu
Issue Date: 29-Jan-2008
Citation: Nagasaki Symposium on Nano-Dynamics 2008 (NSND2008), pp.40-41
Abstract: CEL-III is a hemolytic lectin containing two carbohydrate-binding domains (domains 1 and 2) and a β-sheet-rich domain (domain 3). In domain 3, there is a hydrophobic region containing two α-helices (H8 and H9) and a loop between them, in which alternate hydrophobic residues, especially Val residues, are present. Synthetic peptides corresponding to the loop and second α-helix (H9) showed the strongest antibacterial activity. The recombinant glutathione S-transferase (GST)-fusion proteins containing domain 3 or the α-helical region peptide formed self-oligomers, whereas mutations in the alternate Val residues in the α-helical region lead to decreased oligomerization ability of the fusion proteins. These results suggest that the α-helical region, particularly its alternate Val residues are important for its oligomerization.
Description: Nagasaki Symposium on Nano-Dynamics 2008 (NSND2008) 平成20年1月29日(火)於長崎大学 Poster Presentation
URI: http://hdl.handle.net/10069/9845
Type: Conference Paper
Appears in Collections:Nagasaki Symposium on Nano-Dynamics 2008 (NSND2008)

Citable URI : http://hdl.handle.net/10069/9845

All items in NAOSITE are protected by copyright, with all rights reserved.

 

Valid XHTML 1.0! Copyright © 2006-2015 Nagasaki University Library - Feedback Powerd by DSpace